IVL Swedish Environmental Research Institute

Samples of sediment from two localities subject to discharge of bleachery effluents were enriched continuously with high molecular weight (>l000) fractions from C- and E-stage liquors. After enrichment, bacteria were isolated on a selection of defined media containing substrates structurally related to that of lignin. In total, 18 pure strains were obtained from the E-stage enrichments, 28 from C-stage. 17 of the first and 2 of the second group together with 3 miscellaneous strains were examined for their ability to degrade ether-extracted liquors to tri- and tetrachloroguaiacols (or catechols) and to bring about degradation of 4:5:6 trichloro- and tetra-chloroguaiacol,2:4 dichloro-phenol, 2-chloro-, 2:4-dichloro- and 3:5-dichloro-4-hydroxy-benzoate.Of the 12 strains examined, only three showed any metabolic activity towards the three monomeric substrates tested. All of these strains brought about degradation of the monomeric substrates albeit at differing rates. None of the strains, however, showed any capability of producing polychloroguaiacols or catechols from the C- and E-stage liquors. For the latter, however, evidence was found for the formation of a metabolite similar to tetracholorcatechol

Samples of sediment from two localities subject to discharge of bleachery effluents were enriched continuously with high molecular weight (>l000) fractions from C- and E-stage liquors. After enrichment, bacteria were isolated on a selection of defined media containing substrates structurally related to that of lignin. In total, 18 pure strains were obtained from the E-stage enrichments, 28 from C-stage. 17 of the first and 2 of the second group together with 3 miscellaneous strains were examined for their ability to degrade ether-extracted liquors to tri- and tetrachloroguaiacols (or catechols) and to bring about degradation of 4:5:6 trichloro- and tetra-chloroguaiacol,2:4 dichloro-phenol, 2-chloro-, 2:4-dichloro- and 3:5-dichloro-4-hydroxy-benzoate.Of the 12 strains examined, only three showed any metabolic activity towards the three monomeric substrates tested. All of these strains brought about degradation of the monomeric substrates albeit at differing rates. None of the strains, however, showed any capability of producing polychloroguaiacols or catechols from the C- and E-stage liquors. For the latter, however, evidence was found for the formation of a metabolite similar to tetracholorcatechol